Coding

Part:BBa_M10063:Design

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-10)


{CPompX!}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 175
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Followed designs from Bessette et al, 2004 (PMID: 15531628)


Source

PCR from genomic DNA

References